
Slide a 3D Cas9-guide RNA ribonucleoprotein along a target DNA double helix, lock onto the golden 5'-NGG-3' PAM motif to unwind an R-loop bubble, cleave both DNA strands, and compare NHEJ gene knockout with HDR precise editing.
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This 3D model shows DNA as two antiparallel sugar-phosphate strands wound into a right-handed helix, with adenine-thymine and guanine-cytosine pairs drawn as colored rods. The Unzip fork slider opens the helix from the top, and Auto replicate adds lighter new bases to each template strand. Students can hide hydrogen bonds, change rotation speed, and jump between Whole helix, Fork and Top down views. Use it to teach base pairing rules, the major and minor grooves, and why replication copies information accurately.
Yes. It is completely free and runs in any modern browser, including interactive whiteboards, tablets and Chromebooks. There is nothing to install and no sign-up is needed.
Adenine pairs only with thymine, joined by 2 hydrogen bonds, and guanine pairs only with cytosine, joined by 3. Because of this, one strand always predicts the sequence of its partner.
Drag the Unzip fork slider to open the helix, or turn on Auto replicate. New complementary bases appear as lighter rods on each template strand. Try the Fork camera view for a close look.
About 10 base pairs make one full turn, and each pair adds roughly 0.34 nanometers of length, so one turn is about 3.4 nm. The helix is about 2 nm wide.
Drop it into your LMS, blog, or class site — free.
<iframe src="https://kiwibee.io/en/simulations/dna-double-helix-3d-simulation/embed" title="DNA Double Helix | KiwiBee" loading="lazy" allowfullscreen style="width:100%;height:min(70vh,620px);min-height:420px;border:0;border-radius:16px"></iframe>Continue exploring

Slide a 3D Cas9-guide RNA ribonucleoprotein along a target DNA double helix, lock onto the golden 5'-NGG-3' PAM motif to unwind an R-loop bubble, cleave both DNA strands, and compare NHEJ gene knockout with HDR precise editing.

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From model to evidence
Use a worksheet to capture predictions, observations, and explanations after students explore.
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